Pro-uPA activating protease in plasmas of healthy subjects

Roemisch and colleagues found that about 9 % of a cohort of 140 normal donor plasmas had significant reduced pro-uPA activity while FSAP antigen levels were normal. When FSAP was purified from such plasmas it displayed reduced pro-uPA activating activity, while Factor VII activating activity was normal. In 1 % of the plasmas reduced activity levels as [...]

Marburg I polymorphism of FSAP impairs pro-urokinase activating activity

The FSAP gene contains several single nucleotide polymorphisms. In donors with normal FSAP protein level but reduced pro-uPA activating activity, the mutant allele (SNP 1601) which leads to the G511E amino acid exchange of the mature protein was found. This G511E mutant has been termed FSAP Marburg I (FSAP MI). In this report Roemisch and colleagues use [...]

Factor VII-Activating Protease (FSAP) has Nucleosome-Releasing Factor Activity

The removal of late apoptotic cells is a central step in physiological processes. Plasma proteases appear to be involved by catalyzing the release of nucleosomes from such cells. Molecules having this activity have also been described as “Nucleosome-Releasing Factor” (NRF). Taken together, Zeerleder et al. provide evidence that FSAP may function as a Nucleosome-Releasing Factor. This hints [...]

Detection of a novel serine protease in plasma – later termed FSAP

Hunfeld and colleagues describe the purification of a novel serine protease from human plasma. The molecule was termed “Plasma Hyaluronan Binding Serine Protease (PHBSP, HABP2)”. N-terminal sequencing revealed that it is a heparan-growth factor-like molecule. Later, when its Factor VII activating activity had been detected, it was renamed to “Factor VII-Activating Protease (FSAP)”. Authors and Source: Hunfeld et [...]

Plasma Hyaluronan Binding Protein – later found to be identical with FSAP

In these four publications Choi-Miura and colleagues describe the isolation and characterization of the so-called “hyaluronan-binding protein” from human plasma: later this protein was found to be identical with FSAP. Basically, the 1996 publication of Choi-Miura and colleagues is the first description of FSAP. Authors and Source: Choi-Miura et al. J Biochem. 1996 Jun;119(6):1157-1165 Title: Purification and characterization of [...]